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vitro m1 polarization model  (ATCC)


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    ATCC vitro m1 polarization model
    Mac@WZB117 reverses macrophage <t>polarization</t> imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling <t>M1</t> and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.
    Vitro M1 Polarization Model, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 3572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitro+m1+polarization+model/M-1/pmc13178792-71-2-15
    Average 97 stars, based on 3572 article reviews
    vitro m1 polarization model - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy"

    Article Title: Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy

    Journal: Journal of Extracellular Vesicles

    doi: 10.1002/jev2.70294

    Mac@WZB117 reverses macrophage polarization imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling M1 and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.
    Figure Legend Snippet: Mac@WZB117 reverses macrophage polarization imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling M1 and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

    Techniques Used: Flow Cytometry, Selection, Marker

    Glycolytic reactivation partially attenuates the therapeutic effects of Mac@WZB117. (A) Changes in macrophage phenotype proportions, FACS results showed an increased proportion of M2 macrophages and a decreased proportion of M1 macrophages in the rescue group, indicating reversal of macrophage polarization. (B) qPCR data showed iNOS expression was upregulated, while ARG1 and Mrc1 expression was downregulated, confirming that phenotype remodelling depends on glycolysis inhibition. (C) Haemodynamic parameters (RVSP and mPAP) were significantly elevated after rescue treatment, approaching levels observed in the PAH model. (D) Representative histological staining images and quantitative analysis showed that vascular WT% and α‐SMA‐positive area were markedly increased in the rescue group, suggesting partial reversal of pulmonary vascular structural remodelling. Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.
    Figure Legend Snippet: Glycolytic reactivation partially attenuates the therapeutic effects of Mac@WZB117. (A) Changes in macrophage phenotype proportions, FACS results showed an increased proportion of M2 macrophages and a decreased proportion of M1 macrophages in the rescue group, indicating reversal of macrophage polarization. (B) qPCR data showed iNOS expression was upregulated, while ARG1 and Mrc1 expression was downregulated, confirming that phenotype remodelling depends on glycolysis inhibition. (C) Haemodynamic parameters (RVSP and mPAP) were significantly elevated after rescue treatment, approaching levels observed in the PAH model. (D) Representative histological staining images and quantitative analysis showed that vascular WT% and α‐SMA‐positive area were markedly increased in the rescue group, suggesting partial reversal of pulmonary vascular structural remodelling. Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

    Techniques Used: Expressing, Inhibition, Staining

    Related Articles

    In Vitro:

    Article Title: Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy
    Article Snippet: .. An in vitro M1 polarization model was established using the murine macrophage cell line RAW264.7 (ATCC, TIB‐71). .. Cells were seeded in 6‐well plates at a density of 1 × 10 6 cells/mL and, after adherence, were stimulated for 24 h in RPMI‐1640 medium (containing 10% foetal bovine serum [FBS]) supplemented with 100 ng/mL LPS (Sigma) and 20 ng/mL IFN‐γ (PeproTech) to induce M1 phenotype polarization.



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    vitro m1 polarization model  (ATCC)
    97
    ATCC vitro m1 polarization model
    Mac@WZB117 reverses macrophage <t>polarization</t> imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling <t>M1</t> and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.
    Vitro M1 Polarization Model, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitro+m1+polarization+model/M-1/pmc13178792-71-2-15
    Average 97 stars, based on 1 article reviews
    vitro m1 polarization model - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Mac@WZB117 reverses macrophage polarization imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling M1 and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

    Journal: Journal of Extracellular Vesicles

    Article Title: Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy

    doi: 10.1002/jev2.70294

    Figure Lengend Snippet: Mac@WZB117 reverses macrophage polarization imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling M1 and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

    Article Snippet: An in vitro M1 polarization model was established using the murine macrophage cell line RAW264.7 (ATCC, TIB‐71).

    Techniques: Flow Cytometry, Selection, Marker

    Glycolytic reactivation partially attenuates the therapeutic effects of Mac@WZB117. (A) Changes in macrophage phenotype proportions, FACS results showed an increased proportion of M2 macrophages and a decreased proportion of M1 macrophages in the rescue group, indicating reversal of macrophage polarization. (B) qPCR data showed iNOS expression was upregulated, while ARG1 and Mrc1 expression was downregulated, confirming that phenotype remodelling depends on glycolysis inhibition. (C) Haemodynamic parameters (RVSP and mPAP) were significantly elevated after rescue treatment, approaching levels observed in the PAH model. (D) Representative histological staining images and quantitative analysis showed that vascular WT% and α‐SMA‐positive area were markedly increased in the rescue group, suggesting partial reversal of pulmonary vascular structural remodelling. Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

    Journal: Journal of Extracellular Vesicles

    Article Title: Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy

    doi: 10.1002/jev2.70294

    Figure Lengend Snippet: Glycolytic reactivation partially attenuates the therapeutic effects of Mac@WZB117. (A) Changes in macrophage phenotype proportions, FACS results showed an increased proportion of M2 macrophages and a decreased proportion of M1 macrophages in the rescue group, indicating reversal of macrophage polarization. (B) qPCR data showed iNOS expression was upregulated, while ARG1 and Mrc1 expression was downregulated, confirming that phenotype remodelling depends on glycolysis inhibition. (C) Haemodynamic parameters (RVSP and mPAP) were significantly elevated after rescue treatment, approaching levels observed in the PAH model. (D) Representative histological staining images and quantitative analysis showed that vascular WT% and α‐SMA‐positive area were markedly increased in the rescue group, suggesting partial reversal of pulmonary vascular structural remodelling. Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

    Article Snippet: An in vitro M1 polarization model was established using the murine macrophage cell line RAW264.7 (ATCC, TIB‐71).

    Techniques: Expressing, Inhibition, Staining